isabelwyss164 isabelwyss164
  • 08-01-2024
  • Biology
contestada

A gene does not contain the necessary restriction enzyme sites for cloning into a plasmid vector. What is a possible option?

a. Increase the amount of gene used.
b. Add linkers to generate new restriction enzyme sites.
c. Use a cosmid as a cloning vector.
d. Use dideoxy sequencing to obtain the sequence of the gene.
e. Cut the DNA with a blunt end cutter.

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